精索静脉曲张对青春期大鼠生精细胞凋亡相关基因Bcl-2和Bax表达的影响

Effect of Varicocele on Expression of Apoptosis Associated Gene Bcl-2 and Bax in Spermatogenic Cells of Adolescent Rats

目的研究精索静脉曲张(VC)对青春期大鼠睾丸生精细胞凋亡相关基因Bcl-2/Bax表达的影响,探索精索静脉曲张致不育的机理。方法通过部分结扎左肾静脉建立青春期大鼠实验性精索静脉曲张模型,分别于术后2周、4周、8周取双侧睾丸,用免疫组化SABC法检测Bcl-2/Bax基因表达。结果随着精索静脉曲张时间的延长,Bcl-2表达呈下降趋势,曲张组比对照组低,左侧睾丸比右侧睾丸低;而Bax表达呈增加趋势,曲张组比对照组高,左侧睾丸比右侧睾丸高。结论实验性精索静脉曲张可明显改变青春期大鼠睾丸组织生精细胞凋亡相关基因与Bcl-2/Bax表达。

Objective To study the effect of varicocel(VC) on expression of apoptosis associated gene Bcl-2 and Bax in spermatogenic cells of adolescent rats, and investigate the mechanism of the infertility resulting from varicocele. Methods The varicocele model was created by partial ligation of the left renal vein in the adolescent rats, two and four and eight weeks after creation of the varicocele model,the expressions of Bcl-2 and Bax were detected by immunohistochemical method. Results With the duration of varicocele model, the Bcl-2 expression in experiment group was decreased as compared with that in control group. And in experiment group, the Bcl-2 expression level was lower in left testis than in right testis. But Bax expression in experiment group was increased as compared with that in control group, and in experiment group, the Bax expression level was higher in left testis than in right testis. Conclusion Experimental varicocele could change obviously expression of apoptosis associated gene Bcl-2 and Bax in spermatogenic cells of adolescent rats.