抗人膀胱癌/抗VEGF双功能基因抗体对膀胱癌生物学行为的影响

Bispecific gene antibody(Anti-human Bladder Carcinoma/Anti-VEGF) on animal model of bladder carcinoma

目的:探讨抗人膀胱癌/抗VEGF双功能基因抗体对人膀胱癌生长及转移的影响。方法:通过构建人膀胱癌裸鼠皮下移植瘤模型并注射双功能抗体,观察肿瘤生长及盆腔淋巴结转移情况,同时采用免疫组化法检测肿瘤微血管密度值及凋亡的肿瘤细胞指数。结果:肿瘤大小:双抗组为(19.50±4.51)mm2,对照组为(57.62±8.31)mm2,两组比较P<0.01;肿瘤微血管密度双抗组为(2351±207)个,对照组(4356±548)个,两组相比P<0.05;凋亡指数双抗组为19.25,对照组为9.31,两者比较P<0.05;双抗组无一只发生盆腔转移,而对照组为75.0%,两组比较P<0.05。以上各项组间差异均有统计学意义。结论:抗人膀胱癌/抗VEGF双功能基因抗体对人膀胱癌具有良好的靶向性,能够通过抑制肿瘤微血管形成和加速肿瘤细胞凋亡,遏制实验性人膀胱癌的生长转移,为该抗体用于临床膀胱癌的治疗提供了一定的实验基础。

Objective:To study whether bispecific gene antibody(anti-human bladder carcinoma/anti-VEGF) can inhibit growth of human experimental bladder carcinoma.Methods:Bispecific antibody was injected to nude mice nearby xenograft tumor of human bladder transitional cell carcinoma.Tumor size was measured at different times.Microvessel density,pelvic metastasis,and apoptotic cells in tumor were detected.Results:Tumor size of experiment group was 19.504.51,while that of control group was 57.628.31.Microvessel density of experiment group was 2351207,while that of control group was 4356548.Apoptotic index of experiment group was 19.25,while that of control group was 9.31.There was no pelvic metastasis in experiment group,while metastasis rate of control group was 75.0%.Difference was significant.Conclusions:Bispecific gene antibody can suppress xenograft tumor growth of human bladder transitional cell carcinoma by antiangiogenesis and apoptosis.Experimental base is provided for clinical use.