^(103)钯支架诱导犬胆管增殖平滑肌细胞凋亡与防治胆管狭窄关系研究

Relation of Apoptosis Muscular Cell in ^(103)Pd Radioactive Stent of Dog Biliary Muscular Formation and Inhibition of Biliary Ductal Stricture

目的探讨103钯(103Pd)放射性支架诱导犬胆管增殖平滑肌细胞凋亡与防治犬胆管损伤后胆管狭窄的关系。方法将12只犬随机均分为103Pd支架组和普通支架组,然后将103Pd放射性支架和普通支架分别植入2组犬的肝外胆管内,30d处死全部动物,取出胆管标本,用凝胶电泳和免疫组化染色进行凋亡细胞检测,并用计算机图像检测系统检侧两组胆管腔面积。结果103Pd支架组犬胆管组织中出现明显增殖平滑肌细胞凋亡,而且犬肝外胆管无明显狭窄;普通支架组犬胆管增殖平滑肌细胞凋亡不明显,而且犬肝外胆管有明显狭窄。结论103Pd放射性支架通过促进犬胆管增殖平滑肌细胞凋亡,从而抑制犬肝外胆管损伤后的胆管狭窄。

Objective To research relation of apoptosis muscular cell in ^(103)Pd radioactive stent of dog biliary muscular formation and inhibition of biliary ductal stricture. Methods Twelve dogs were randomly divided into two groups, which were general stent group and ^(103)Pd radioactive stent group. General stent and ^(103)Pd radioactive stent were respectively put into extrahepatic biliary tract of two groups. After 30 days all dogs were killed, and biliary tract were taken out. Apoptotic cells were detected by immunohistochemical methods and agar electrophoresis, and nucleus brow-yellow was positive cell. Dog biliary duct cross-sections were stained by hematoxylin- erosin; area and perimeter of lumen,thickness of inner membrane and stenosis degree in bile duct were analysed by image analysis software of computer.Results The apoptotic biliary duct smooth muscle cell [(87.9±7.96)/cm^2] was more significantly increased in the ^(103)Pd radioactive stent group than in the general stent group [(5.6±0.51)/cm^2], P<0.05; and comparing with the general stent group, the ^(103)Pd radioactive stent significantly reduced biliary muscular formation thickness. Conclusion The result shows that ^(103)Pd radioactive stent can inhibit proliferation of biliary ductal smooth muscle cell.