肝细胞MDR1基因表达与胆囊胆固醇结石相关性研究

Study on the Relationship Between MDR1 Gene Expression in Liver Cell and Cholesterol Calculus in Gallbladder

目的探讨肝细胞中MDR1基因的表达与胆囊胆固醇结石形成之间的关系。方法应用逆转录聚合酶链反应(RT PCR)技术,检测胆囊胆固醇结石患者和正常对照人群肝细胞标本MDR1mRNA的表达水平。结果以β2微球蛋白为内参照,胆固醇胆囊结石患者肝细胞中MDR1mRNA表达水平低与对照组(1.30±0.19vs2.25±0.28),二者之间的差异具有显著性意义(P<0.01)。结论胆囊胆固醇结石的形成可能与肝细胞中MDR1基因的表达降低有关。

Objective To explore the relationship between the expression of MDR1 gene in liver cell and the formation of cholesterol calculus in gallbladder.Methods The mRNA expression level of MDR1 gene in liver cell of the cholesterol calculus group and the normal control group were measured through reverse transcriptionpolymerase chain reaction (RT-PCR), and microglobulin β_2 was used as internal contrast.Results The MDR1 mRNA expression level of the cholesterol calculus group was lower than that of the normal control group(1.30±0.19 vs 2.25±(0.28), P<0.01). Conclusion The formation of cholesterol calculus in gallbladder is related to the reducd expression level of MDR1 gene in liver cell.