摘要
目的观察血管紧张素Ⅱ(AngⅡ)刺激心肌细胞,造成心肌细胞肥大过程中组蛋白脱乙酰基酶2(histonedeacetylase2,HDAC2)的表达。方法培养原代心肌细胞,AngⅡ刺激心肌细胞造成肥大,在不同时间取细胞进行反转录聚合酶链反应(RT-PCR),观察HDAC2mRNA表达,免疫组化法检测HDAC2蛋白表达,相差镜和电镜下观察细胞形态变化。结果经AngⅡ刺激后,相差镜下可见心肌细胞面积变大;电镜下见细胞内部结构亦发生明显的改变;HDAC2mRNA水平随着AngⅡ刺激时间延长而增高;HDAC2蛋白表达亦增加。结论AngⅡ致心肌细胞肥大过程中伴有HDAC2表达增加,HDAC2有可能参与心肌细胞的肥大机制。
Objective To observe the expression of histone deacetylase 2 (HDAC2) in hypertrophiccardiocytes induced by angiotensin Ⅱ (AngⅡ). Methods The cultured cardiocytes were treated with AngⅡ toinduce hypertrophy. In different periods, the mRNA levels of HDAC2 were examined by real-time PCR method. Theprotein expressions of HDAC2 were examined by immunohistochemistry method. The morphologic changes ofcardiocytes were observed under the contrast phase microscope and electron microscope. Results The myocyteswere enlarged under the contrast phase microscope and the inner structure also changed accordingly under electronmicroscope. After stimulated by AngⅡ , the mRNA level and protein expression of HDAC2 increased in thehypertrophic cardiocytes. Conclusions The expression of HDAC2 increases in hypertrophic cardiocytes stimulatedby AngⅡ. HDAC2 may play a role in cardiocyte hypertrophy.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2005年第4期392-395,共4页
Chinese Jouranl of Endemiology
基金
黑龙江省自然科学基金资助项目(D01-51)
