摘要
目的建立大鼠新鲜骨组织基因组DNA的提取方法。方法取大鼠肋骨,研磨成粉末,脱钙,水洗,蛋白酶K消化,有机溶剂抽提,离心沉淀干燥,-20℃冰箱中保存。结果提取的骨组织基因组DNA纯度较高,数量大,性质稳定。结论本提取方法,简便易行,获得的基因组DNA量大,纯度高,值得推广。
Objective To establish a method of extracting genome DNA from fresh rat bone. Methods Ratribs were ground to fine powder, decalcified, washed by water and digested by protease K followed by extractionwith organic solvent, centrifugal and sedimentary dehydration and storage at a - 20 ℃ refrigerator. ResultsGenome DNA extracted by the method was of high purity and large quantity, the character of genome DNA wasstable. Conclusions The method is easy to handle, by which genome DNA is extracted in a good amount and ahigh purity. The method is worth of recommendation.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2005年第4期449-451,共3页
Chinese Jouranl of Endemiology
基金
国家自然科学基金资助项目(30371245)
陕西省科学技术研究发展计划项目(2004K12-G12)
陕西省自然科学研究计划(2001SM82)
关键词
骨组织
基因组
DNA
骨脱矿质技术
Bone tissue
Genome
DNA
Bone demineralization technique