抗核小体抗体在系统性红斑狼疮诊断中的应用

Significance of Anti-Chromatin Antibodies in Diagnosis of Systemic Lupus Erythematosus

目的:探讨抗核小体抗体(AnuA)在系统性红斑狼疮(SLE)诊断中的敏感性和特异性,及其与疾病活动的相关关系。方法:用酶联免疫吸附法(ELISA)检测SLE和其他风湿病患者及健康对照者血清中AnuA、抗ds-DNA抗体的含量,用系统性红斑狼疮疾病活动指数(SLEDAI)对狼疮患者的病情进行评价,并分析其与AnuA的相关性。结果:AnuA在SLE患者中的阳性率为84%,较ds-DNA抗体的阳性率(66%)高(χ2=4.92,P<0.05)。AnuA含量与SLEDAI相关。SLE肾炎患者AnuA水平较SLE非肾炎患者高(χ2=26.37,P<0.05),AnuA阳性SLE患者肾炎发生率较AnuA阴性者升高。结论:AnuA在SLE中的阳性率高,可以用于监测SLE的病情变化。

Objective To investigate the specificity and the sensitivity of anti-chromatin antibodies for the diagnosis of systemic lupus erythematosusSLE. Methods Fifty patients with SLE were included in this study and 30 healthy blood donors were included as controls. Disease activity was assessed using the Systemic Lupus Erythematosus Disease Activity IndexSLEDAI. The venous blood sample was drawn to measure 2 anti-chromatin antibodies anti-nucleosome anti-NCS anti-double-stranded DNA anti-dsDNA by enzyme-linked immunosorbent assay. Results The sensitivity of anti-chromatin antibodies in SLE patients was 84% for anti-NCS and 66% for anti-dsDNA. Anti-NCS antibodies showed the highest correlation with disease activity. Anti-NCS antibodies also showed strong association with renal damage. Conclusion Anti-NCS antibodies could be a useful tool in the diagnosis and assessment of disease activity in SLE patients.