摘要
目的:体外观察罗勒多糖对人高转移肺癌细胞侵袭、转移行为的影响,并探讨其可能的作用机制。方法:在体外侵袭模型中观察不同浓度罗勒多糖对人高转移肺癌细胞侵袭、转移行为的影响;划痕标记染料示踪技术检测罗勒多糖对细胞通讯功能的影响;RT-PCR检测肿瘤细胞内转移相关分子c-myc、nm23-H1与Tiam-1mRNA表达水平的改变。结果:罗勒多糖处理组细胞的侵袭与转移能力明显低于对照组(P<0.05);细胞通讯功能有一定程度的恢复,荧光染料传递毗邻3-4列细胞;细胞内促进肿瘤细胞转移的基因c-myc、Tiam-1表达程度低于对照组,而抑制肿瘤细胞转移的基因nm23表达程度高于对照组(P<0.05)。结论:罗勒多糖是一种具有抗人高转移肺癌细胞侵袭以及转移作用的新药,它的作用是通过多个药效靶点综合作用的结果。
AIM: In this paper, we studied the efficiencies and the mechanisms of a new Chinese herb Qcimum basilicum polysaccharide (BP) on PG cell metastasis in vitro. METHODS: The number of tumor cells going through matrigel was assayed and used to represent the ability of the invasion and migration of PG cells. Using Scrape-loading and dye transfer (SLDT) technique, the efficiencies of BP on recovering PG cell gap junction -mediated intercellular communication (GJIC) was measured. The expressions of c-myc, nm23-H1 and Tiam-1 genes mRNA in PG cells treated with BP were determined by RT-PCR. RESULTS: Compared with the control, the action of invasion and migration of PG cells were decreased after treated with BP (P<0.05). The GJIC of PG cells were recovered in different degrees after treated with BP for 8 h. The fluorescence reached some rows of cells from the scraped line. The expressions of c-myc and Tiam-1 genes in PG cells treated with BP were decreased, but the expression of nm23-H1 gene was increased compared with the control. CONCLUSIONS: BP is a new anti-pulmonary cancer cell metastasis reagent. Its antitumor metastasis action could be achieved by recovering the cell GJIC and changing the expression of some metastasis associated genes.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第7期1345-1348,共4页
Chinese Journal of Pathophysiology
基金
山东省卫生厅基金资助项目(No.2001CA1CDA1)
山东省中药管理局基金资助项目(No.99-46)
关键词
罗勒多糖
肿瘤转移
肺肿瘤
Qcimum basilicum polysaccharide
Neoplasm metastasis
Lung neoplasms