体外罗勒多糖抗人高转移肺癌细胞侵袭转移作用及机制探讨

Experiment research of anti-PG cell metastasis and mechanism of a new Chinese herb Qcimum basilicum polysaccharide

目的:体外观察罗勒多糖对人高转移肺癌细胞侵袭、转移行为的影响,并探讨其可能的作用机制。方法:在体外侵袭模型中观察不同浓度罗勒多糖对人高转移肺癌细胞侵袭、转移行为的影响;划痕标记染料示踪技术检测罗勒多糖对细胞通讯功能的影响;RT-PCR检测肿瘤细胞内转移相关分子c-myc、nm23-H1与Tiam-1mRNA表达水平的改变。结果:罗勒多糖处理组细胞的侵袭与转移能力明显低于对照组(P<0.05);细胞通讯功能有一定程度的恢复,荧光染料传递毗邻3-4列细胞;细胞内促进肿瘤细胞转移的基因c-myc、Tiam-1表达程度低于对照组,而抑制肿瘤细胞转移的基因nm23表达程度高于对照组(P<0.05)。结论:罗勒多糖是一种具有抗人高转移肺癌细胞侵袭以及转移作用的新药,它的作用是通过多个药效靶点综合作用的结果。

AIM: In this paper, we studied the efficiencies and the mechanisms of a new Chinese herb Qcimum basilicum polysaccharide (BP) on PG cell metastasis in vitro. METHODS: The number of tumor cells going through matrigel was assayed and used to represent the ability of the invasion and migration of PG cells. Using Scrape-loading and dye transfer (SLDT) technique, the efficiencies of BP on recovering PG cell gap junction -mediated intercellular communication (GJIC) was measured. The expressions of c-myc, nm23-H1 and Tiam-1 genes mRNA in PG cells treated with BP were determined by RT-PCR. RESULTS: Compared with the control, the action of invasion and migration of PG cells were decreased after treated with BP (P<0.05). The GJIC of PG cells were recovered in different degrees after treated with BP for 8 h. The fluorescence reached some rows of cells from the scraped line. The expressions of c-myc and Tiam-1 genes in PG cells treated with BP were decreased, but the expression of nm23-H1 gene was increased compared with the control. CONCLUSIONS: BP is a new anti-pulmonary cancer cell metastasis reagent. Its antitumor metastasis action could be achieved by recovering the cell GJIC and changing the expression of some metastasis associated genes.