人骨髓间质干细胞与新型可降解材料生物相容性的实验研究

Cytocompatibiltiy of degradable calcium metaphosphate with human marrow mesenchymal stem cells

目的:本研究利用人骨髓间质干细胞(MSC)的增殖与分化潜能作为指标,对可降解偏磷酸钙(dCMP)材料和羟基磷灰石(HA)材料的生物相容性进行体外研究。方法:通过扫描电镜观察MSC在dCMP表面粘附的情况,并利用ICP和IC分析dCMP和HA的降解产物元素含量,同时采用FACS、ALP活性检测及ARS等方法对降解产物的毒性效应进行检测。结果:dCMP对MSC的增殖有促进作用,且不影响MSC的成骨分化进程及分化后的矿化功能;而HA对MSC的成骨分化进程无影响,但对MSC的增殖和成骨分化后的矿化功能均有抑制作用。结论:dCMP的生物相容性较HA为佳,更适合作为骨替代材料。

AIM: To screen the cytotoxicity of degradable calcium metaphosphate (dCMP) compared with hydroxyapatite (HA). The proliferation and differentiation abilities of human marrow mesenchymal stem cells (MSC) were used to exhibit the cytotoxicity. METHODS: The cell morphology of MSC was analysed after direct contact with dCMP at different time points by scanning electron microscopy analysis. The degradation products of dCMP and HA were analysed with inductively coupled plasma torch and ion chromatography. The cytotoxic effect of degradation products of dCMP was evaluated by FACS, quantitative assay of ALP and ARS, respectively. RESULTS: dCMP enhanced the proliferation of MSC, but didn't interfere the osteogenic differentiation process of MSC and its mineralization. HA inhibited the proliferation of MSC and the mineralization of osteogenic differentiated MSC, while it did not interfere the osteogenic differentiation process of MSC. CONCLUSION: dCMP had a better cytocompatibility with MSC than HA, which might allow for its use as skeleton scaffolds.