黑熊肌酸激酶及其同工酶活性的检测

Detection of the creatine kinase activity and CK isozyme activity of black bear

目的对圈养的健康四川黑熊血清中的肌酸激酶(CK)及其同工酶CK-MB活性进行测定,建立起黑熊肌酸激酶(CK)及其同工酶CK-MB活性的生化参考指标,为研究黑熊积累资料,并为黑熊的疾病防治提供科学依据.方法根据酶促反应速率原理,结合分光光度法,对CK和CK-MB的活性进行人工检测.对检测结果进行统计分析,求出酶活性的平均值、变异系数和期望值置信区间.结果在30℃条件下,雄性黑熊CK活性平均值为162.70 U/L,变异系数为11.90%,期望值置信区间为148.90～176.50 U/L;CK-MB活性平均值为17.4 U/L,变异系数为17%,期望值置信区间为15.26～19.54 U/L;雌性黑熊CK活性平均值为149.3 U/L,变异系数为8%,期望值置信区间为140.68～157.92 U/L;CK-MB活性平均值为15.8 U/L,变异系数为18.6%,期望值置信区间为13.70～17.90 U/L.结论所得结果可以作为健康黑熊的部分生化指标.

Objective To detect the creatin kinase (CK) and its isozeyme (CK-MB) activity in serum of the healthy black bear (Ursus thibetanus) reared in pens to establish reference standard of biochemistry for CK and CK-MB activities of black bears as well as to offer scientific basis for prevention and cure of the black bear disease. Methods The CK activity and CK-MB activity were measured using spectrophotometer based on the principle of enzymatic reaction rate. All data were used to calculate the mean value, variation coefficient and the confidential interval of expected value of enzymatic activity. Results Under 30 ℃, of the male black bears, the average CK activity is 162.70 U/L, the variation coefficient 11. 90%, the confidential interval of expected value 148.90 to 176.50 U/L, and the average CK-MB activity is 17.4 U/L, the variation coefficient 17%, the confidential interval of expected value 15.26 to 19.54 U/L. Of the female black bears, the average CK activity is 149.3 U/L, the variation coefficient 8%, the confidential interval of expected value 140.68 to 157. 92 U/L, and the average CK-MB active is 15.8 U/L, the variation coefficient 18.6%, the confidential interval of expected value 13.70 to 17.90 U/L. Conclusion The data we obtained can serve as biochemical indexes for the healthy black bears.