大戟内生菌的抑菌活性及其与宿主相关性研究

Correlation between inhibition activity of endophytic fungus from Euphorbia pekinensis and its host.

为了开发利用药用植物的内生菌资源,以大戟和其内生真菌作为材料,以一株乌桕来源的内生菌作为对照,研究自身具有抑菌作用的药用植物的内生菌在抑菌作用上和宿主的相关性.结果表明,大戟内生菌镰刀菌E5有抑制宿主受试病原菌的作用,并在活植物体内表现出对受试病原菌的拮抗作用;其抑菌能力受宿主成分刺激而加强,而宿主成分对受试病原菌无抑制作用,乌桕来源的内生菌链隔孢S12无抑制受试病原菌的作用.内生菌E5需要在氧气充足条件下培养才能产生抑菌作用,抑菌活性最大产生时间和生物量最大值不同时出现.内生菌E5的抑菌能力受到pH、温度、紫外线影响.大戟内生菌E5以上特点,体现了其与宿主之间的互惠共生关系.

To develop endophytic fungi resources from medicinal plants,this paper studied the correlation between endophytic fungus Fusariium sp.E5 from Euphorbia pekinensis and its host through analyzing the inhibition activity of this pathogen.An endophytic strain S12 (Alternaria sp.) from Sapium sebiferum was used as contrast.The results showed that E5 had an inhibitory effect on the host,while S12 and E.pekinensis extract didn't have it.The inhibition activity of E5 could be stimulated by the extract of host leaf,and its inhibitory effect could only be found when it was cultured with enough oxygen.The inhibition activity was affected by pH,temperature,and ultra-violet.These characters of endophytic fungus E5 showed a good correlation with its host,and this correlation was mutualism.