摘要
目的探讨某市生活饮用水的致突变性。方法采用Ames试验和小鼠肝原代细胞单细胞凝胶电泳(彗星试验)分别对某市自来水厂的水源水、出厂水、管网水的有机浓集物的诱变性进行检测。结果出厂水和末梢管网水的Ames试验于试样浓度为3.0L/皿时才表现为阳性结果,而肝细胞的单细胞凝胶电泳在水样量为0.1L时就出现明显的阳性结果(与阴性对照比较,P<0.01),后者的浓度比前者低30倍。水源水的Ames试验于试样浓度为6.0L/皿时仍表现为阴性结果,而原代肝细胞的单细胞凝胶电泳在水样量为0.5L时就出现明显的阳性结果。结论Ames试验只检测出氯化消毒后饮用水的致突变阳性,而单细胞凝胶电泳能同时检测出水源水和氯化消毒后饮用水的致突变阳性。
Objective To have a knowledge of the mutagenicity of contaminates in drinking water in a certain city. Methods Mutagenic activity of organic extracts of source water chloridized water in water plant and tap water from the city were detected with single cell gel-electrophoresis mouse primary hepatocytes in culture were used and Ames test. Results For Ames test the organic extract in 3 liters chloridized water in water plant and tap water samples produced positive result and that in 6 liters source water sample still gave a negative result. For single cell gel-electrophoresis test the organic extract in 0.1 liter chloridized water in water plant tap water and 0.5 liter source water produced positive result. Conclusion The sensitivity of single cell gel-electrophoresis with primary hepatocytes in detection of genotoxicity of cloridized water and source water is much higher than Ames test.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2005年第4期289-291,共3页
Journal of Environment and Health
关键词
水污染
诱变力试验
AMES试验
肝原代细胞
单细胞凝胶电泳试验
Water contamination Mutagenicity test Ames test Primary hepatocytes Single cell gel-electrophoresis