苯甲酸雌二醇对大鼠睾丸组织发育的影响

Influence of Estradiol Benzoate on the Testis Development of Rats

目的:研究苯甲酸雌二醇(E2B)对雄性大鼠睾丸组织发育的影响。方法:选取新生雄性SD大鼠40只,随机均分为实验组和对照组,在生后1d分别皮下注射E2B(0.2mg/5g体重)、等体积的溶剂玉米油,分别于生后14、21、28、42、56d取睾丸并称重。测量头段及尾段生精小管管径(TD)及上皮高度(SEH),并计算头段及尾段TD/SEH比值、尾段SEH/头段SEH比值,分析生精小管内生精细胞发育状况。结果:各年龄段实验组大鼠睾丸重量显著下降(P<0.01),睾丸始终停留在腹腔。睾丸网内始终有液体潴留,从出生后21d开始,实验组大鼠头段睾丸的生精小管的TD/SEH比值明显大于对照组(P<0.01),尾段SEH/头段SEH比值也显著增加(P<0.01);实验组生精细胞发育明显迟滞于对照组(P<0.01)。结论:E2B可以造成雄性大鼠睾丸内液体潴留、隐睾,从而阻滞睾丸组织的正常发育。

Objective: To study the influence of estradiol benzoate (E2B) on the testis development of Sprague-Dawley (SD) rats.Methods: Newborn male SD rats were injected subcutaneously with E2B((0.2) mg/5 g body weight). At days 14, 21, 28, 42 and 56 after birth, the rats were sacrificed after anaesthesia, and their testes were taken out and weighed. The cranial and caudal SEH and TD were measured respectively, the TD/SEH ratio in each part of the testis and the caudal SEH/cranial SEH ratio were calculated, and different stages of spermatogenesis were analysed.Results: In the experimental group, the testis weight was significantly lower than that of the control (P<(0.01)), the testes constantly remained in the abdominal cavity, and there was much fluid retention in the rete testis all the time. From day 21 on, the TD/SEH ratio in the cranial testis was much higher than in the control (P <(0.01)), and so was the caudal SEH/cranial SEH ratio(P<(0.01)). And spermatogenesis was obviously retarded as compared with the control(P<(0.01)).Conclusion: Exposure of newborn male SD rats to E2B can cause fluid retention in the testis and cryptorchid, which in turn may retard spermatogenesis