转化生长因子β_1和基质金属蛋白酶及其组织抑制物在慢性鼻窦炎和鼻息肉组织中的差异性表达

The protein expression difference of transforming growth factor β_1,matrixmetalloproteinases 1,7,9 and tissue inhibitors of matrix metalloproteinases-1 between chronic rhinosinusitis,nasal polyps and normal mucosa tissues

目的:探讨转化生长因子β1(TGF-β1),基质金属蛋白酶1、7、9(MMP-1、7、9)及其组织抑制物-1(TIMP-1)在慢性鼻窦炎和鼻息肉以及正常下鼻甲黏膜组织中表达程度的差异。方法:采用酶联免疫吸附试验(ELISA)检测22例慢性鼻窦炎筛窦黏膜、21例鼻息肉和15例下鼻甲黏膜组织中TGF-β1,MMP-1、7、9以及TIMP-1的蛋白表达量,并比较他们在不同组织中表达程度的差异。结果:①TGF-β1、MMP-7、MMP-9及TIMP-1在慢性鼻窦炎和鼻息肉组织中的表达均比正常下鼻甲黏膜组织增高,差异具有统计学意义(均P<0.05);②慢性鼻窦炎组织中TGF-β1和TIMP-1相比鼻息肉组织中的表达增高,差异具有统计学意义(P<0.01和P<0.05);③鼻息肉组织中MMP-7的表达比慢性鼻窦炎组织中的表达增高,差异具有统计学意义(P<0.01)。结论:慢性鼻窦炎和鼻息肉组织显示出不同的TGF-β1、MMP-7、TIMP-1蛋白表达水平。

Objective:To compare the expression difference of transforming growth factor β_1(TGF-β_1), matrix metalloproteinases1,7,9(MMP-1,7,9 ) and tissue inhibitors of matrix metalloproteinases-1(TIMP-1 ) protein among the chronic rhinosinusitis tissues, nasal polyps tissues and normal nasal mucosa tissues.Method:The protein expression of TGF-β_1, MMP-1, MMP-7, MMP-9 and TIMP-1 in chronic rhinosinusitis tissues from 22 patients, in nasal polyps tissues from 21 patients and in inferior turbinate tissues from 15 patients underwent nasal septum operation was detected by enzyme-linked immunosorbent assay (ELISA).Result:①In chronic rhinosinusitis and nasal polyps tissues,compared with controls,the expression of TGF-β_1,MMP-7,MMP-9,TIMP-1 protein was significant increased(P<0.05);②The expression of TGF-β_1 and TIMP-1 in chronic rhinosinusitis tissues was significant increased versus that in nasal polyps tissues (P<0.05);③Concentration of MMP-7 protein in nasal polyps tissues was found significant higher than that in chronic rhinosinusitis tissues (P<0.01).Conclusion:Different TGF-β_1,MMP-7, TIMP-1 protein level in chronic rhinosinusitis and nasal polyps tissues might be the molecular basis of the different histopathological character of chronic rhinosinusitis and nasal polyp tissues.