人下鼻甲微血管内皮细胞的体外培养及鉴定

Culture and identification of microvascular endotheliocytesfrom human nasal inferior turbinate tissue

目的:研究人下鼻甲微血管内皮细胞的体外培养方法,为鼻腔、鼻窦相关疾病的研究提供实验基础。方法:采用手术中获得的人下鼻甲边缘黏膜及黏膜下组织,机械分离和酶解消化微血管内皮细胞,在体外条件下进行培养。分别行细胞形态学观察、细胞生长曲线的测定和培养细胞免疫荧光鉴定。结果:体外培养条件下得到人下鼻甲微血管内皮细胞,经细胞形态学观察、细胞生长曲线测定及免疫荧光鉴定证实为血管内皮细胞。结论:本研究获得的体外培养人下鼻甲微血管内皮细胞,可为鼻腔过敏性疾病和炎症性疾病的微血管病理改变及药物对鼻腔下鼻甲微血管功能影响等研究提供实验基础。

Objective:To study the method of culturing human nasal inferior turbinate endotheliocytes in vitro,and to provide the study base for nasal-related disease.Method:The endotheliocytes from human nasal inferior turbinate were gotten in nasal surgery and cultured in vitro.Result:We got human nasal inferior turbinate microvascular endotheliocytes in vitro, and confirmed them by immunity fluorescence.Conclusion:Human nasal inferior turbinate microvascular endotheliocytes can be cultured in vitro, and be used in the studies of nasal-related disease.