细菌性阴道病实验室诊断方法的比较

Camparison of Methods for Diagnosing Bacterial Vaginosis

【目的】比较细菌性阴道病的4种实验室诊断方法。【方法】随机选择就诊病人100例分别用Amsel法,组织多胺试验,一步法唾液酸酶活性的检测试验,革兰染色细菌评分法检测细菌性阴道病。【结果】Amsel法阳性率28%,组织多胺试验阳性率24%,一步法唾液酸酶活性的检测试验阳性率34%,革兰染色细菌评分法阳性率30%;唾液酸酶活性检测法与Amsel法,革兰染色细菌评分法相比较差异无显著性;组织多胺试验与Amsel法,革兰染色细菌评分法相比较差异无显著性;以Amsel法为标准,一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为92.9%和71.4%,特异性分别为88.9%和94.4%,阳性预期值分别为76.5%和83.3%,阴性预期值分别为97.0%和89.5%;以革兰染色细菌评分法为标准,一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为93.8%和66.7%,特异性分别为92.1%和94.3%,阳性预期值都为83.3%,阴性预期值分别为97.2%和86.8%。【结论】一步法唾液酸酶活性的检测试验和组织多胺试验快速,简便,易于标准化,可以用于临床诊断细菌性阴道病。

[Objective]To compare the value of one step sialidase,Amsel,Gram-stain and amines method in diagnosis of bacterial vaginosis.[Methods]The methods of Amsel, one step sialidase,amines and Gram-stain were performed simultaneously to diagnose bacterial vaginosis in 100 women.[Results]Positive rate of Amsel was 28%, one step sialidase was 34%, Gram-stain was 30% and amines was 24%.Using Amsel as criterion, the sensitivity of one step sialidase and amines was 92.9% and 71.4%;specificity was 88.9% and 94.4%;positive predictive value was 76.5% and 83.3%;negative predictive value was 97% and 89.5%.using Gram-stain method as criterion, the sensitivity of one step sialidase and amines was 93.8% and 66.7%, specificity was 92.1% and 94.3%,positive predictive value was 83.3% and 83.3%, negative predictive value was 97.2% and 86.8% respectively.[Conclusion]The detection of one step sialidase and amines can be used to diagnose bacterial vaginosis,as they are convenient and easily standardized methods.