宫颈癌患者病毒感染及其细胞因子水平变化的调查研究

Relationship of Virus Infection, Soluble Interleukin 2 and Tumor Necrosis Factor in Patients with Cervical Cancer

用PCR和ELISA技术对195例宫颈癌和75例宫颈良性疾病患者组织标本进行病毒病原体和血清SIL2R、TNF的测定。结果宫颈癌患者组织中HPV16、18、35型检出率为31.79%(62/195);HSVⅡ为30.77%(60/195);HCMV为3.08%(6/195),均高于良性疾病患者的20%、4.0%、6.6%和0,经χ2检验差异显著,均P<0.01。而HPV6.11型、EBV、Uu及CT的检出率在良恶性两组之间比较,差异无显著性,均P>0.05。宫颈癌患者血清SIL2R和TNF水平明显高于良性疾病组,且宫颈癌病毒感染阳性者血清SIL-2R和TNF水平明显高于阴性者,经统计学处理差异显著,均P<0.01。结论HPV16、18、35型、HSVⅡ和HCMV合并感染及血清中TNF和SILIR水平明显升高,可能是山西地区宫颈癌高发的一个重要因素。

Objective To study on the relationship of virus infection, Tumor Necrosis (TNF) and Soluble Interleukin 2 Receptor (SIL-2R) levels in patients with cervical cancer. Methods Virus infection were detected by Polymerase Chain Reaction(PCR) and the levels of TNF,SIL-2R were measured by Enzyme Linked Immunosorbent Assay (ELISA) in 195 cases cervical cancer, and 75 cases benign diseases. Results The result showed that 152 in 195 cervical cancer cases were infected by virus (positive rates 77.95%). The positive rates of Human Popillomavirus type 16,18,35(HPV_ 16,18,35), Herpes Simplex Virus type 2 (HSVⅡ) and Human Cytomegalovirus (HCMV) were 31.% (62/195), 30.76% (60/195) and 3.08% (6/195) respectively. Its positive rates were significantly higher than that of the 20%(15/75), 4.0% (3/75), 6.67% (5/75) and zero from 75 cases begin disease, (P< 0.01, respectively). The result also showed the positive rates and levels of SIL-2R and TNF were significantly higher in virus infection than that of the uninfection patients with cervical cancer (P< 0.01, respectively). Conclusion These results suggest that the mixture infection of HPV_ 16,18,35, HSVⅡ and HCMV may play the cooperation role in inducing cervical cancer. The findings indicated virus infection may cause disorder and deficiency of immunity function, and variation of cytokines levels.