阿霉素诱导人肝癌细胞凋亡机制的研究

A study on the mechanism of adriamycin-induced apoptosis of human hepatic carcinoma cells

探讨阿霉素诱导HCC-9204细胞凋亡的作用机制,及其与凋亡相关基因Bcl-2和Bax表达的关系。方法用TUNEL法和流式细胞仪观察和检测阿霉素对HCC-9204细胞增殖周期的影响以及细胞凋亡,并检测HCC-9204细胞中Bcl-2和Bax蛋白的表达水平。结果阿霉素能够显著诱导HCC-9204细胞凋亡,染色显示有典型的凋亡特征。在药物处理24h后流式细胞仪检测出显著的“亚二倍体峰”。阿霉素作用HCC-9204细胞后,Bcl-2蛋白表达明显减少(P<0.05);而Bax蛋白的表达虽有所增加,但与对照组相比差异无显著性(P>0.05)。结论阿霉素能诱导癌细胞凋亡,是其发挥抗癌作用的重要机制之一,这可能与其下调Bcl-2基因表达有关。

ObjectiveTo investigate the mechanism of adriamycin-induced apoptosis of hepatic carcinoma HCC-9204 cells, and study the expression of apoptosis-associated genes Bcl-2 and Bax. MethodsTUNEL method and flow cytometry were used to detect the effect of adriamycin on the multiplication cycle of human hepatic cancer ACC-9204 cells, and the cell apoptosis. The expression of Bel-2 and Bax protein in (ACC-9204) cells was examined. ResultsAdriamycin markedly induced apoptosis of HCC-9204 cells, and typical characteristics of apoptosis were detected by staining. On cell cytometric analysis 24h after drug (treatment), a significant sub-G1 peak was detected. HCC-9204 cells treated with adriamycin showed (significant) down regulation of Bel-2 protein expression, but it was not significantly different from that of the control group(P>0.05). ConclusionsAdriamycin can induce apoptosis of cancer cells, and this is an important mechanism for its anticancer effect. This effect may be related to the down regulation of Bel-2 (expression).