摘要
用滇龙胆的顶芽及侧芽为外植体,以MS培养基为基本培养基,以6-BA/NAA和KT/IAA两组不同浓度组合进行对比实验,研究诱导滇龙胆顶芽及侧芽萌发产生不定芽的最佳条件。发现在诱导率、产生的不定芽数、长势及不定芽出现时间等方面上,BA/NAA两种激素的互作用效果明显优于KT/IAA。其中以MS+BA3.0mg/l+NAA1.5—2.0mg/l的培养基上诱导率较高,可达100%,且小植株生长茁壮,叶色浓绿,大部分在10d左右可萌发出不定芽,产生不定芽数达7—9个。
The indefinite bud was induced from terminal bud and lateral bud by using different hormone 6-BA/NAA and KT/IAA in the tissue culture of Gentiana rigescens Franch..ex Hemsl and MS culture (medium) as the basic culture medium. The hormone BA/NAA has obvious advantages over KT/IAA in terms of inductive rate, amount of indefinite bud, growth vigor and time for occurrence of indefinite buds. Moreover, BA/NAA are obviously superior to KT/IAA in terms of interplay effect.
出处
《楚雄师范学院学报》
2005年第3期49-52,共4页
Journal of Chuxiong Normal University
关键词
滇龙胆
组织培养
不定芽
顶芽
侧芽
研究
<Keyword>Gentiana rigescens Franch..ex Hemsl
tissue culture
indefinite bud
terminal bud
lateral bud
study
