摘要
利用Ｇ－ＣＳＦ依赖型细胞株Ｎ８Ｆ－６０，尝试将ＭＴＴ比色法应用于基因工程产品重组人粒系集落刺激因子（ｒｈＧ－ＣＳＦ）的活性检测。多次实验探索出最适的细胞浓度和培养时间，细胞数为低于１×１０￣４／孔，培养时间为３０─４８ｈ。在宽的线性范围内检测Ｇ－ＣＳＦ样品的生物活性，结果经传统的ＣＦＵ－Ｇ方法验证可靠，且较后者更灵敏、简便、省时、省力，并可定性、定量地检测样品活性。特别适用于大量样品的同时检测。另外，此方法可避免接触放射性。
The G-CSF dependent cell NSF-60 MTT method was established to measure thebioactivity of rhG-CSF. Under the most appropriate conditions of cell number≤1×10￣4 / well,culture time 30─48 hours,etc,very good results were obtained.This method is more sensitive,simple and rapid as compared with CFU-G method.It is suitable for examining a large numberof samples at the same time,and can avoid the radioactivityin the￣3H-TdR incorporationmethod.
出处
《中国应用生理学杂志》
CSCD
1995年第3期280-283,共4页
Chinese Journal of Applied Physiology
