山丹启动培养中生理生化特性的研究

The Research on the Physiologic and Biochemical Characters in the Startup Culture Process of the Low Lily

研究了山丹百合鳞茎不同部位鳞片在启动培养过程中可溶性糖、淀粉、可溶性蛋白质、游离氨基酸等几种内含物及POD、SOD酶活性的变化,探讨组培过程中内在生理生化的变化规律与百合离体再生培养器官分化之间的关系,结果表明:山丹启动培养中POD、SOD的酶活性的高低与外植体分化率的大小有一定的相关性。可溶性糖、可溶性蛋白质和淀粉在外植体启动培养初期主要被用于提供分化所需养分,其含量的高低与组织分化率关系不很明显。

In this research, the bulbils from various parts of the low lily(Lilium pumilum DC) were being studied upon the variation of its content materials such as sugar, amylum, protein, amino acid and the enzyme activities of the POD, SOD etc in its startup culture process. The relationship between the internal physiological-biochemical varying regulations in the tissue culture process and the organs differentiation in the body-segregation culture of the low lily were also being sought for. The results indicated that: in the startup culture process of the low lily, the enzyme activity degrees of the POD, SOD were relatively correlated with the differentiating ratio of the explants. During the initial start-up culture stage of explants, the soluble sugar, protein and amylum were mainly used to provide nutrition for its differentiation, and their concentration degree had no significant correlations with the tissue differentiation ratio.