亲水/亲脂性附加剂对乳酸-羟乙醇酸共聚物微球中蛋白释放的影响

Effect of hydrophilic/hydrophobic additives on protein release from PLGA microspheres

目的考察亲水/亲脂性附加剂对乳酸-羟乙醇酸共聚物(PLGA)微球中蛋白释放的影响。方法采用复乳-溶剂挥发法制备PLGA微球,并添加不同性质的附加剂考察蛋白的释放行为。微量BCA法测定蛋白质量浓度。结果加或不加附加剂对PLGA微球的粒径无显著影响,对微球包封率的影响较大。加入羟丙β-环糊精使蛋白从PLGA微球的释放明显增加;硬脂酸则使蛋白释放明显减缓;聚乙二醇则对蛋白释放有一定的阻滞作用。它们的扫描电镜图发生了相应的改变。结论加入亲水/亲脂性附加剂可修饰PLGA微球中蛋白的释放。

OBJECTIVE: To investigate the effects of hydrophilic/hydrophobic additives on protein release: from PLGA microspheres. METHODS: The conventional PLGA microspheres and PLGA microspheres with one of additives, HP-β-CD, PEG 6000 or stearic acid, were all prepared by the double emulsion solvent evaporation method. Their protein contents of bovine serum albumin (BSA) were determined by micro-BCA method. RESULTS: No significant effects of the additives on the particle sizes were observed. Additives affected the protein encapsulations apparently. Hydrophilic HP-β-CD obviously accelerated the release of BSA from the PLGA microspheres, Hydrophobic stearic acid significantly decreased the release of protein, and amphiphilic PEG 6000 delayed the protein release at some degree. Their SEM showed the corresponding changes. CONCLUSION: The protein released from PLGA microspheres can be modified by adding into different hydrophilic/hydrophobic additives.