高效液相色谱法测定西酞普兰血药浓度

Determination of citalopram in human plasma by high-performance liquid chromatography

目的建立高效液相色谱法测定西酞普兰血药浓度。方法采用液-液萃取法,用正己烷-异戊醇(98:2)提取血浆中西酞普兰,选用普萘洛尔为内标。色谱柱为NucleodurCN(4.6mm×250mm,5μm),流动相:乙腈-30mmol·L-1KH2PO4缓冲液(0.1%三乙胺,pH5.0)(40:60),激发波长:236nm,发射波长:306nm,流速:1.3ml·min-1,柱温:室温。结果本法线性范围在1-75μg·L-1(r=0.9996)。平均方法回收率为(96.8±5.0)%(n=15),日内RSD≤8.6%,日间RSD≤9.6%。结论本法简单、快速、准确,可用于临床血药浓度监测及人体药动学研究。

OBJECTIVE: To establish a HPLC method for the determination of citalopram in human plasma. METHODS: A liquid-liquid extraction method was used. Citalopram in plasma was extracted with hexane-isoamyl alcohol (98:2), and propranolol was used as the internal standard. The sample was separated on a Nucleodur CN (4.6 mm × 250 mm,5 μm) column, with a mobile phase of 30 mmol&middotL -1 potassium dihydrogenphosphate buffer (0.1%TEA,pH 5.0)-acetonitrile (60:40), and citalopram was detected by a fluorescence detector with an excitation wavelength of 236 nm and an emission wavelength of 306 nm. The flow rate was 1.3 mL&middotmin-1 and the column temperature was room temperature. RESULTS: The linear range of citalopram was 1-75 ng&middotmL -1 (r = 0.999 6). The recovery of assay was (96.8 ± 5.0)% (n = 15). The coefficients of variation within day and between days did not exceed 8.6% and 9.6% , respectively. CONCLUSION: The method-appeared to be simple, convenient and precise for plasma drug level monitoring and clinical pharmacokinetic study of citalopram.