高效聚磷菌株GM1的分离和聚磷特性研究

ISOLATION AND CHARACTERIZATION OF STRAIN GM1 WITH HIGH CAPABILITY OF ACCUMULATING POLY-P

采用纯培养结合蓝白斑筛选法从土壤中筛选到一株高效聚磷菌,初步鉴定为费氏柠檬酸杆菌属(Citrobacterfreundii),命名为GM1。GM1在LB、YG和MOPS培养基上均可正常生长,其生长pH在5·5至8·5之间,最适生长pH为7·5。该菌在不同的培养基上最适生长温度不同,在LB培养基上为37℃,YG和MOPS-葡萄糖培养基上为30℃。在好氧条件下MOPS培养基培养24h后,GM1菌体含磷量为11·5%;上清液磷浓度由43·8mgL-1下降为14·7mgL-1,磷去除率达69%,poly-P染色显示菌体中有异染粒。GM1具有较强的聚磷能力。

A strain of bacteria with high capability of accumulating poly-P was isolated and screened from soils by using the pure culture and blue- and white-colored screening methods. Identified as Citrobacter freundii and named GM1,this strain could grow normally in either LB,YG or MOPS media with pH ranging from 6.5 to 8.5. The optimal temperature and pH for its growth were 30℃ and pH 7.5,respectively. After incubation aerobically in the MOPS medium for 24 hours,the phosphorus concentration in GM1 reached 11.5% of its weight,while the concentration of phosphate in the medium declined from 43.8 to 14.7 mg L~ -1,so the phosphate removing rate was 69%. At that time,poly-P granules could be observed in thalli by the poly-P staining method,indicating that GM1 has a high capability of accumulating phosphate.