大豆对斜纹夜蛾抗性的遗传分析及相关QTL的定位

Inheritance Analysis and Mapping QTLs Related to Cotton Worm Resistance in Soybeans

以大豆组合皖82-178×通山薄皮黄豆甲衍生的重组自交系群体(RIL)为材料,以斜纹夜蛾幼虫重为抗性鉴定指标,应用主基因+多基因的混合遗传模型对大豆抗虫性进行遗传分析。结果表明,该群体对斜纹夜蛾的抗性遗传符合两对主基因+多基因的混合遗传模型,主基因的遗传率为89.85%。以该群体构建的遗传连锁图谱为基础,利用软件CartgrapherV.2.0采用复合区间作图法检测到2个与抗虫有关的QTL,分别位于wt-11和wt-12连锁群上,其在对应连锁群的端距离分别为5.51cM和11.51cM,加性效应估计值分别为-0.0619和-0.0419,对性状变异的解释率分别为17.22%和8.60%。

This study employed the soybean recombinant inbred lines (RIL) which involved 133 lines from Wan82-178× Tongshanbopihuangdoujia as experimental materials. Larval weight of cotton worm [Prodenia litura (L.) Fabricius] was examined and used as an index of resistance. The inheritance of soybean resistance to cotton worm was estimated by major gene plus polygene mixed model. The result indicated that the resistance was controlled by two major genes plus polygene, and the heritability value of major genes is 89.85%. Based on the linkage map constructed with SSR markers of this RIL population, two QTLs related to the trait were mapped on linkage group wt-11 and wt-12, and the position were 5.51 cM and 11.51 cM, respectively. The QTLs’ additive effects were –0.0619 and –0.0419 and explained 17.22% and 8.60% of the total variation of the larval weight, respectively.