10味间糜栓阻断宫颈癌发生的临床及实验研究

The Clinical and Experimental Research of Shiwei Jianmi Shuan on Cervical Carcinoma Blick

10味间糜栓治疗宫颈重度糜烂457例,痊愈377例,占82.49％;无效6例,占1.31％,总有效率98.68％,治疗宫颈非典型增生31例,合并原位癌者8例,经病理证实30例转变为慢性宫颈炎,1例有效。对宫颈非典型增生患者治疗前后宫颈细胞DNA含量与核面积测定结果显示,它能使DNA合成速率降低,S期细胞数明显减少,阻断非典型增生细胞由G_1期向S期的进程,细胞核面积缩小。与实验研究流式术测定U_(14)宫颈癌结果相同。抗肿瘤作用的整体观察,说明它能明显提高小鼠的生命延长率,与阳性对照组无差异。组织和细胞培养的研究,肯定了该栓剂无选择性地直接杀伤细胞,似同抗癌西药丝裂霉素的作用。抗肿瘤细胞化学研究,证实它能直接破坏癌细胞DNA和RNA,损坏正在分裂中期的染色体。抑菌实验证实对阴道常见的6种病菌,均具有很强的抑制作用。

457 cases with severe cervical erosion were treated with Shiwei Jianmi Shuan. 377 cases were cured, the cure rate was 82. 49% ; six cases failed to respond to the treatment, the inefficacy rate was 1. 31%. The total efficiency rate is 98. 68%. 31 cases of atypical cervical hyperplasia were treated. Among them, 8 patients suffered from carcinoma in situ. All patients were cured. Cervical dysplasias were changed to chronic cervicitis, which was confirmed by pathological examination. The DNA contents and the nuclear area of the cells with atypical cervical hyperplasia were measured before and after treatment. The results showed that the rate of DNA synthesis of cervical cells, the number of S-phase cells and the nuclear area of the cells were reduced. The transition of cells with atypital cervical hyperplasion from G1 phase to S phase were inhibited. The results were same as that of the experiment in which U14 cervical carcinoma was detected by the flow cy-tometry. The general observation of antitumor effect disclosed that Shiwei Jianmi Shuan could raise the life prolonged rate of mice, and had no difference with positive control group. The research of the suppository by the method of tissue and culture confirmed that the suppository could directly kill the cancer cells with no choice, which was similar to the Mitomycin (the anti-cancer drug). The results of chemical research into the antineoplastic effect of the suppository showed that it could directly decompose the DNA and RNA of cancer cells, and decompose chromsome in the middle phase of mitosis. The bacteriological tests verified that the suppository had a strong inhibiting effect on the six common bacteria of the vagina.