孚尔根整体染色法在动物组织学研究中的应用

Application of FEULGEN Whole Response Staining Method to Animal Histology Study

孚尔根整体反应,使组织块中细胞核呈紫红色,为组织学制片简化了操作步骤.制片再经固绿复染后细胞质呈淡绿色,核质红绿对比染色鲜明而清晰美观.

In the article is reported the application of”FEULGEN Whole Response”to animal histology study. The experi- ment is soaking piece tissues into 1 mol hydrochloric acid under the condition of 60℃, hydrolyzing soaked piece tissues at different time, then staining the samples with XIFU reagent as well at different time. It shows that tissue slice will come into best play when the piece tissue has been soaked in 1 mol hydrochloric acid for 3 hours at the room temperature and hydrolyzed 10 minutes at 60℃, while the best effect of XIFU Reagent staining will be got 24 hours after. Through FEULGEN Whole Response, the color of kary- on becomes amaranth. This method has simplified the procedure for making histology tissue slices. After piece tissues are re - stained by green color, the cytoplasm’s color will turn pale green, which makes karyon easily observed from cytoplasm, and the whole picture clear and beautiful.