高效液相色谱法测定人血清中头孢呋辛浓度

Determination of Cefuroxime in human serum by HPLC

作者建立了快速、简便测定人血清中头孢呋辛反相高效液相色谱法（ＨＰＬＣ法），采用５×２００ｍｍ不锈钢柱，内填１０μｍＹｗＧ－Ｃ＿１８Ｈ＿３７固定相，流动相甲醇：水：冰醋酸（２８：７１：１ｖ／ｖ），流速１．２ｍｌ／ｍｉｎ，以头孢噻肟为内标物，紫外检测波长２９０ｎｍ，用１０％三氯醋酸作蛋白沉淀剂，与血清之比为１：２（Ｖ／Ｖ）。头孢呋辛的日内平均相对回收率为１００．３４％±６．６０％，日间平均相对回收率为９９．６９％±６．３３％。头孢呋辛最低检测限为２ｎｇ，最低检测浓度为０．２μｇ／ｍｌ，在０．２５～１０μｇ／ｍｌ度范围内线性良好，相关系数ｒ＝０．９９９９。

Yu Chen(Xu Hui District Central Hospital,200031)A rapid and convenient quantitative methed was reported for the determination of Cefuroxime in human plasma by re-versed phase HPLC.A ￠5 × 2000mm steel colume fitted with 10μm YWG-C_18H_37 was used. The mobile phase was consistedof mthanol:purified water :acetic acid (28 :71:lv/v)and the flow rate was 1.2ml/min.Claforan was used as an internalstandard. The detection was carried out at 290nm.The 10%trichloroacetic acid was used for the sedimentation of protein andthe ratio to plasma was 1 :2(v/v).The average relative recoveries of withinday were 100.34%±2.69%. The daverage rel-ative recoveries of between day were 99.69%±2.31%. The detection limit of cefurodime was 2ng or 0.2μg/ml in plasma.The method was linear within the range of 0.25 to 10μg/ml and the coefficient of corrlation was 0.9999.