新疆察吾呼沟古代居民线粒体DNA序列多态性分析

Analysis of Mitochondrial DNA from the Ancient Tombs of Chawuhu

从距今2500~3000年的新疆察吾呼沟墓地的9例古代人骨中成功地提取出古DNA分子.用4对套叠引物对线粒体DNA高可变Ⅰ区进行了PCR扩增和测序,得到9个364bp的线粒体序列.从GenBank搜索其共享序列并与欧洲、亚洲序列进行系统发育分析,结果表明,早在青铜至早期铁器时代,在我国新疆天山中部地区已经有蒙古人种存在.察吾呼沟古代居民应是一个欧洲和东亚人种混合的古代群体.

The DNA was successfully extracted from the human bone samples excavated from the ancient tombs of Chawuhu, which has been dated back to (2 500)(3 000) years ago according to the feature of pottery and a series of ^(14)C data. Part of the mitochondrial hypervariable region (364 bp) was amplified and sequenced with four overlapping primers. In order to search their sharing sequences and determine their pedigree, each sequence was searched and compared with the database GenBank. Phylogenetic analysis indicated that there existed the admixture of Euro-Asian lineage in the east of Xinjiang prior to the time of the Bronze Age.