薄层荧光扫描法对清肝片中含量测定

Determination of HDCA and CDCA of Qinggan Pill with Thin-layer Fluorescence Scanning

目的:建立清肝片中猪去氧胆酸(HDCA)及鹅去氧胆酸(CDCA)的含量测定方法。方法:在硅胶G板上以环己烷-醋酸乙酯-醋酸-甲醇(20∶25∶2∶3)展开,以薄层荧光扫描法,激发波长为366nm,测定清肝片中HDCA及CDCA的含量。结果:平均回收率分别为HDCA:99.9%,RSD=1.9%;CDCA:100.9%,RSD=2.8%。结论:该方法灵敏、准确,适于作为清肝片的质控标准。

Objective: to decide the methods of determining HDCA and CDCA of Qinggan Pill. Methods: to unfold cyclohexane, cerin, acetic ether and methanol (20∶25∶2∶3) on silica G pallet to determine the content of HDCA and CDCA by thin-layer fluorescence scanning with excitation wave of 366 nm. Results: The average recovery rates of HDCA and CDCA were respectively 99.9% and 100.9%and the RSD were respectively 1.9% and 2.8%. Conclusion: The method is sensitive and accurate and can be used as quality control standard for the pill.