听源性癫痫发作对大鼠学习记忆的影响及机制探讨

Mechanism of epileptic attack on learning and memory in rats

目的探讨癫痫发作对大鼠学习记忆的影响及机制。方法以100dB铃声刺激60s诱发遗传性听源性癫痫大鼠癫痫发作,采用光辨别学习和放射免疫分析相结合的方法,研究大鼠癫痫发作对光辨别学习的影响及脑组织中生长抑素含量的改变。结果癫痫发作后,大鼠光辨别学习能力明显减弱,表现为达到学会标准所需的时间明显延长(6.20±0.97vs3.90±0.57,P<0.01);癫痫发作后,大鼠下丘脑、海马、颞叶皮层组织中生长抑素含量明显增高(下丘脑:551.28±68.21vs273.41±53.51,P<0.05;海马:246.71±43.26vs135.74±36.60,P<0.01;颞叶皮层:235.88±42.14vs150.01±37.71,P<0.01)。结论癫痫发作对大鼠光辨别学习有明显的抑制作用,该作用可能与下丘脑、海马及颞叶皮层组织中生长抑素含量的改变有关。

ObjectiveTo study the effect of epilepsy on light discrimination learning and to explore the possible mechanism. Methods100dB ringing evokes audiogenic epilepsy on epilepsy-prone rats. To explore the effect of epilepsy on light discrimination learning and somatostatin (SS) contents by means of behavior and radioimmunoassay. ResultsThe ability of light discrimination learning weakened significantly after epilepsy seizure, evidenced by the prolonged time to learn to meet the standard ( P <0.01);the SS in hypothalamus, hippocampus and cerebral cortex increased significantly after epilepsy seizure( P <0.01).ConclusionEpilepsy seizure attenuate the learning ability of rats. This effect may be related to the alteration of SS in hypothalamus, hippocampus and cerebral cortex.