高效液相色谱法同时测定普罗帕酮、氢化奎尼丁的血药浓度

A HPLC method for simultaneous determination of propafenone and hydroquinidine in human serum

本文建立了用高效液相色谱法同时测定普罗帕酮、氢化奎尼丁血药浓度的方法。所用色谱柱为ＯＤＳ柱，流动相为改性甲醇，检测波长为２５４ｎｍ，流速１．２ｍｌ／ｍｉｎ，内标物采用盐酸异丙嗪，用氯仿作为提取剂，提取后分离有机层，于５０℃水浴以氮气流吹干，残渣用甲醇重溶，取１０μｌ进样。以样品峰面积对内标峰的峰面积比对样品浓度进行直线回归，计算直线回归方程，线性范围普罗帕酮为０．１～８．０ｍｇ／Ｌ，氢化奎尼丁０．５～６．０ｍｇ／Ｌ；普罗帕酮和氢化奎尼丁的最低检出限分别为：５ｎｇ和２．５ｎｇ，日内误差ＲＳＤ％分别为：４．３９％和３．８２％，日间误差ＲＳＤ％分别为：４．８３％和３．６３％。普罗帕酮、氢化奎尼丁和异丙嗪的绝对回收率分别为９８．３１％、９２．１７％和８７．１７％。本法方便、快速、灵敏、准确，特别适用于常规临床血药浓度监测。

A method for simultaneous determination of propafenone and hydroquinidine serum concentration was de-scribed using a reversed phase column,packed with C18(5μm)and UV detection at 254nm,phenergan as internalstandard.These drugs were extracted from 0.5 ml serum with 5 ml chloroform,evaporated under nitrogen at 50℃water bath, and reconsitituted in methanol.The extracted samples were chromatographed using 500 ml methanoladded 1 ml glacier acid and 0.5 ml n-propylamine as mobile phase,at flow rate of 1.2ml/min. The absolute recov-eries of propafenone,hydroquinidine and phenergan were 98. 31%, 92.17%and 87.17%, respectively.The lin-eary range from 0.5～6.0mg/L for hydroquinidine and 0.1～8.0mg/L for propafenone. The detection limit were2.5 ng for hydroquinidine and 5 ng for propafenone. The method is easy and rapid to handle,has high sensitivityand accuracy; is especially suitable for TDM.