新疆雪莲细胞悬浮系的建立和黄酮类活性成分的产生

Cell suspension culture and flavonoids production in Saussurea involucrate

目的:建立新疆雪莲的悬浮培养体系。方法:研究不同培养基、培养基初始pH、碳源、接种量、植物生长物质含量对雪莲细胞生长和黄酮合成的影响。结果:N6液体培养基在pH5.8、蔗糖含量50g·L-1、接种量60-80g·L-1FW时有利于细胞生长和黄酮合成,附加BA0.2mg·L-1+NAA2mg·L-1有利于黄酮合成,而附加BA0.5mg·L-1+NAA3mg·L-1则对生长有利。结论:培养条件的优化可有效提高雪莲悬浮细胞的生长和总黄酮的合成。

Objective: To establish a system of cell suspension culture for Saussurea involucrate. Method: The effects of different factors on cell growth and flavonoids production of S. involucrate were systematically studied including the media, initial pH values of the medium, carbon sources, inoculum quantity, and plant growth substance. Result: The optimum medium was N6, initial pH values of the medium was5. 8, sucrose concentration was 50g·L-1, inoculum quantity was 60-80 g·L-1 FW. Medium supplemented with BA (0.5mg·L-1) and NAA(3 mg·L-1) was suitable for cell growth, but medium containing BA (0. 2 mg·L-1) and NAA(2 mg·L-1) was suitable for flavonoids production. Conclusion: Cell growth and flavonoids production in the suspention of S. involucrate culture cell should be optimized.