失血性休克再灌注大鼠血液NO、NOS的改变和丹参的作用

The Changes of NO and NOS in Rats with Hemorrhagic Shock/reperfusion and the Effect of Salvia Miltiorrhiza (SM)

目的探讨失血性休克再灌注大鼠血液中一氧化氮(NO)、一氧化氮合酶(NOS)含量的变化及丹参对其的影响。方法健康SD大鼠30只,随机平分成正常对照组(NC组)、失血性休克再灌注组(HS-R组)和丹参治疗组(SM组)。分别测定三组血清NO、NOS含量。结果与NC组相比较,HS-R组血清NO、NOS含量明显增高(P<0.01)。SM组的血清NO、NOS含量明显低于HS-R组(P<0.01)。结论失血性休克再灌注时NOS大量激活,使NO产生增多,NO介导了失血性休克再灌注损伤,大量释放的NO参与休克再灌注损伤的脂质过氧化反应;丹参通过减少NO的生成,抗脂质过氧化反应而发挥其保护作用。

Objective To investigate the changes of NO and NOS in rats with hemorrhagic shoek/ reperfusion and the effect of salvia miltiorrhiza. Methods 30 SD rats were randomly divided into three groups: normal control (NC) group (n = 10), hemorrhagic shock/reperfusion(HS - R) group( n = 10) and salvia miltiorrhiza(SM) group ( n = 10) .To estimate the value of NO and NOS in plasma of every group in the end of experiment. Results In HS - R group, the contents of NO and NOS in plasma were increased remarkable compared to NC group( P < 0.01) . In SM group, the contents of NO and NOS were markedly lower than those in HS - R group ( P < 0. 01 ) . Conclusion These results suggest that NO and NOS were increased remarkable in hemorrhagic shock/reperfusion. NO may be involved in oxidant - mediated shock/reperfusion. SM play protection on hemorrhagic shock/reperfusion injury through decreasing the generation of NO and anti - 1 Lipid peroxidation.