烟草制丝过程中游离氨基酸的变化研究

Study on variety of free amino acids in tobacco by reversed-phase high performance liquid chromatography in primary processing

超声波水解提取烟叶中的游离氨基酸、AccQ-2A氨基酸衍生化试剂衍生化、反相高效液相色谱法测定卷烟制丝过程中松片回潮、润叶加料、烘丝三个工序前后烟丝中的游离氨基酸。结果表明:制丝加工制丝过程中A、B两个不同牌号的16种游离氨基酸总量增加,幅度分别达32.5%和22.1%。

During cigarette process as tobacco leaf strip loosing & humidifying, wetting & casing and drying, The free amino acids in tobacco were extracted by ultrasonic, then derived with AccQ-2A and separated by reversed-phase high-performance liquid chromatography. The experiments demonstrate that after processing, the total contents of free amino acids in cut tobacco increased remarkably, by 32.5% (of brand A) and 22.1% (of brand B).