4－甲基组胺和三氟啦嗪对WEHI_3细胞增殖的影响

THE EFFECTS OF 4-MH AND TFP ON THE PROLIFERATION OF WEHI_3 CELL

用体外细胞液体培养法研究组胺Ｈ＿２受体激动剂４－甲基组胺（４－ＭＨ）、Ｈ＿２受体拮抗剂西咪替丁和钙调素拮抗剂三氟啦嗪（ＴＦＰ）对小鼠粒单白血病ＷＥＨＩ＿３细胞增殖的影响。结果表明：４－ＭＨ呈剂量依赖性抑制ＷＥＨＩ＿３细胞增殖。１０￣（－４）ｍｏｌ／Ｌ西咪替丁可阻断这种效应。１０￣（－５）─１０￣（－４）ｍｏｌ／ＬＴＦＰ阻断ＷＥＨＩ＿３细胞增殖，１０￣（－６）ｍｏｌ／ＬＴＦＰ对细胞影响不大，但与高浓度４－ＭＨ合用有协同抑制效应。

The effects of 4-MH(4-methylhistamine,an agonist of histamine H_2-receptor),cimetidine(an antagonist of histamine H_2-receptor)and TFP(trifluoperazine, a calmodulin antagonist)onthe proliferation of WEHI_3 cells(a myelomonocytic leukemia cell line)were investigated in theliquid culture in vitro.It was shown that 4-MH at concentrations ranging from 10￣(-7)mol/L to 10￣(-5)mol/L significantly inhibited the proliferation of WEHI_3 cells (P<0.05).These inhibitoryeffects were displayed in a dose-dependant manner and were completely blocked by the additionof cimetidine at concentration of 10￣(-4)mol/L,TFP caused a dose-dependent inhibition of theproliferation of WEHI_3 cells at concentrations ranging from 10￣(-5)mol/L to 10￣(-4)mol/L, but didnot affect the growth of WEHI_3 cells at the low concentrations(<10￣(-5)mol/L).TFP at lowconcentration(10￣(-6)mol/L) exhibited synergetic inhibitory effect with high concentrations of4-MH(>10￣(-5)mol/L)on the proliferation of WEHI_3 cells. The above findings suggested thatTFP and 4-MH could inhibit the proliferation of WEHI_3 cell and 4-MH could influence theproliferation of WEHI_3 cell by stimulating histamine H_2 receptor. Calmodulin probably wasinvolved in the mechanism of growth regulation of WEHI_3 cells by 4-MH.