摘要
采用低浓度胰蛋白酶,较低温度下长时间搅拌及粗口径滴管分次吹打等改进方法进行新生大鼠骨骼肌细胞分散培养,能够得到较高的细胞产率。经10nmol/L秋水仙碱处理3d后可得到肌球,能够适合于进行膜片钳记录,并不改变肌细胞的电生理学特性,肌球膜上有正常的Na ̄+、K ̄+和N型乙酰胆碱受体离子通道活动。本方法提供了一个较好的适用于肌细胞电生理学研究的模型。
Low
concentration of trypsin,prolonged time of dissociation with stirring
at low tempera-ture and gentle trituration with large-diameter
pipettes were adopted to culture skeletal musclecells from neonatal
rats.The rate of live cells was high.Three days after treatment with
10nmol/L colchicine, spherical myoballs formed spontaneously from
myotubes.Myoballs couldbe used in patch-clamping experiments
especially in whole-cell-recording mode,The colchicinehad no apparent
effect on the electrophysiological properties of the muscle
cells.There were nor-mal Na ̄ +,K ̄and N type ACh receptor ion
channels in the membrane of myoball.The cultureof myoballs could be a
suitable preparation for patch,clamping studies on cultured rat
skeletalmuscles.
出处
《中国应用生理学杂志》
CAS
CSCD
1995年第1期83-86,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金
关键词
细胞培养
肌球
膜片钳
骨骼肌
cell culture
myoball
patch-clamping
AChR
single channel