摘要
本实验研究了不同浓度和不同作用时间的去甲肾上腺素(NA)对大鼠脾脏T淋巴细胞增殖的影响,并探讨α和β受体在此影响中的作用。结果表明,10 ̄(-8)--10 ̄(-5)mol/LNA可显著抑制由刀豆素A(ConA)诱导的T细胞增殖反应,其中10 ̄(-7)mol/LNA的抑制作用最强。NA与已被ConA刺激lh的T细胞作用后,或者NA与脾细胞作用1h后被洗去,T细胞的增殖仍受到抑制,但是NA不能抑制已被Con A刺激6h的T细胞的增殖。α受体拮抗剂酚妥拉明(Phen,10 ̄(-6)mol/L)和β受体拮抗剂心得安(Prop,10 ̄(-7)mol/L)均可完全阻断10 ̄(-8)和10 ̄(-7)mol/LNA的免疫抑制作用。结果提示,NA具有非浓度依赖性的细胞免疫抑制作用,此作用可能发生于T细胞激活前或激活早期,并可能通过α和β受体介导。
The effect of
noradrenaline(NA)on spleen T lymphocyte proliferation induced
byconcanavalin(Con A) was studied with the aid of MTT colorimetric
assay.The proliferation of Tcells did not change when T cells were
incubated simultaneously with 10 ̄-(10)or 10-(9)mol/L NAand Con A.
However, NA at 10(-8)to 10(-5)mol/L dose range significantly
inhibited theproliferation of T cells induced by Con A and the
strongest inhibition occurred at 10 ̄(-7)mol/LNA. The T cell
proliferation still decreased when NA(10 ̄(-8)to 10 ̄(-6)mol/L) was
added to T cellshaving stimulated by Con A for an hour and incubated
for 72 hours or when NA was washed offafter one-hour incubation with
T Cells which were then incubated with Con A for 72 hours,butthe T
cell proliferation did not change when NA was added to Tcells having
incubated with ConAfor 6 hours.The NA(10 ̄(-8)or 10 ̄
(-7)mol/L)suppression ofTcell proliferation could be blockedby either
α-adrenergic antagonist phentolamine orβ-adrenergic antagonist
propranolol.It issuggested that NA can cause
concentration-independent inhibition of T cell proliferation,andthe
inhibitory effect of NA may occur either befor activation of T cells
or just after stimulationof T cells by Con A and may be mediated by
α-and β-adrenergic receptors.
出处
《中国应用生理学杂志》
CAS
CSCD
1995年第1期75-78,共4页
Chinese Journal of Applied Physiology
关键词
去甲肾上腺素
神经递质
细胞免疫
T细胞
noradrenaline
T-lymphocyte
neurotransmitter
cellular
immunity,neuroimmunomo-dulation