运动性疲劳状态下大鼠心肌线粒体内膜变化的研究

CHANGES OF INNER MITOCHONDRIAL MEMBRANE OF MYOCARDIUM DURING EXERCISE-INDUCED FATIGUE

采用递增负荷力竭性运动模型，观察了Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ大鼠急性运动至力竭后心肌线粒体内膜流动性、ＮＡＤＨ－ＣｏＱ还原酶及ＡＴＰ酶活性的变化。结果表明，大鼠心肌线粒体内膜荧光偏振值较安静时显著增高（Ｐ＜０．０１），示膜流功性降低。线粒体内膜ＮＡＤＨ－ＣｏＱ还原酶和ＡＴＰ酶活性分别较安静时下降６１．６％和７４．３％（Ｐ＜０．０５，Ｐ＜０．０１）。提示，力竭性运动后大鼠心肌线粒体内膜功能改变，其膜流动性和呼吸链酶活性变化，可能是运动性疲劳的重要膜分子机制之一。

Rats running with an incremental intensity to exhaustion was used as an experimental modelto investigate the effect of acute exhaustive exercise on inner mitochondrial membrane ofmyocardium. It was found that,in exhausted rats,fluorescence polarization and membranemicroviscosity of inner mitochondrial membrane increased significantly following the exereise;the activities of NADH-CoQ reductase and ATPase in respiratory chain declined by 61.1%and 74.3%,respectively.The results suggest that the functional alterations in mitochondrial mem-brane and changes in activity of enzymes of respiratory chain may be the membrane molecularmechanism of exercise-induced fatigue.