前列腺素F_（2α）对大鼠肾小球系膜细胞DNA合成的影响

INFLUENCE OF PROSTAGLANDIN F_(2α) ON DNA SYNTHESIS IN RAT MESANGIAT CELLS

本实验应用［￣３Ｈ］胸腺嘧啶核苷（￣３Ｈ－ＴｄＲ）掺入法测定前列腺素Ｆ＿（２α）（ＰＧＦ＿（２α））对大鼠肾小球系膜细胞ＤＮＡ合成的作用，测定系膜细胞合成的二脂酰甘油（ＤＡＧ）及磷酸肌醇（ＩＰ）。结果表明，ＰＧＦ＿（２α）促进系膜细胞的ＤＮＡ合成、ＤＡＧ及ＩＰ的生成。提示ＰＧＦ＿（２α）使系膜细胞的磷脂酶Ｃ活化，产生ＩＰ及ＤＡＧ，激活蛋白激酶Ｃ，从而促进ＤＮＡ合成及细胞增殖。

DNA synthesis, production of diacylglycerol (DAG) and inositol phosphates(IP)were de- termined in cultured rat mesangial cells exposed to prostaglandin F_(2α)(PGF_(2α)).PGF_(2α) dose-dependently enhanced DNA synthesis assessed by ￣3H-thymidine incorporation in quies- cent mesangial cells.PGF_(2α) also stimulated the production of DAG and IP in mesangial cells. These results suggest that PGF_(2α) can promote DNA synthesis and proliferation of mesangial cells through activation of phospholipase C and protein kinase C.