反复癫痫发作对大鼠光辨别学习的影响及机制探讨

THE EFFECT OF REPEATED EPILEPSY SEIZURES ON LIGHT DISCRIMINATION LEARNING AND MECHANISM

本研究在遗传性听源性癫痫大鼠模型上，采用了行为和放射免疫分析相结合的方法，研究了大鼠反复癫痫发作对光辨别学习的影响及心脑组织中Ｐ物质（ＳＰ）含量的改变。结果表明：（１）随癫痫发作频率增高，大鼠光辨别学习能力明显减弱。表现为达到学会标准所需时间明显延长（Ｐ＜０．０１），达标率明显降低（Ｐ＜０．０１）。（２）癫痫发作后，大鼠下丘脑和海马中ＳＰ含量明显降低（Ｐ＜０．０１），而颞叶皮层和心肌组织ＳＰ含量明显增高（Ｐ＜０．０５─０．０１）。（３）随着癫痫发作频率增高，大鼠光辨别学习能力下降的同时，下丘脑和海马中ＳＰ含量明显降低，而颞叶皮层和心肌组织中ＳＰ含量随癫痫发作次数增加呈先上升后下降现象（Ｐ＜０．０５─０．０１）。结果提示，癫痫发作次数增加对大鼠光辨别学习有明显的抑制效应，该效应可能与下丘脑、海马、颞叶皮层及心肌组织中ＳＰ含量的改变有关。

The effect of epilepsy seizure on light discrimination learning and the substance P(SP)con-tents of brain and heart were studied by means of behavior and radioimmunoassay in audiogenicepilepsy model of genetically epilepsy-prone rats.The results were as follows:(1)The ability oflight discrimination learning was significantly weakened with increasing frequency of epilepsy sei-zures,as indicated by prolongation of the time required to reach the criterion of acquisition(P<0.01).(2) The SP contents were significantly decreased in hypothalamus and hippocampus(P<0.01), while increased in cerebral cortex and myocardium after epilepsy seizure(P<0.05─0.01).(3)While the ability of learning of the rats was weakened with increasing frequency of epi-lepsy seizures,the SP content was significantly decreased in hypothalamus and hippocampus, andfirst increased then decreased in cerebral cortex and myocardium. The results indicate that epilep-sy seizure weakens the learning ability of rats and the effect may be related to SP inhypothalamus,hippocampus,cerebral cortex and myocardium.