命门合剂对大鼠神经内分泌网络的影响

Effect of Ming-Men Mixture on Corticosterone-Rats' Neuro-Endocrino-Immunological Network

为了研究中医命门或肾与神经内分泌免疫网络的本质联系，用皮质酮（ＣＯＲＴ）皮下注射塑造大鼠下丘脑－垂体－肾上腺－胸腺轴（ＨＰＡＴ）抑制模型，观察命门合剂对大鼠神经内分泌免疫网络的调节作用。结果表明：模型组大鼠下丘脑室旁核小细胞区促肾上腺皮质激素释放因子（ＣＲＦ）阳性神经元及正中隆起ＣＲＦ阳性神经纤维，垂体前叶促肾上腺皮质激素（ＡＣＴＨ）阳性细胞等明显减少，免疫组织化学ＡＢＣ法染色变浅；肾上腺萎缩特别是束状带变薄；胸腺萎缩，淋巴细胞与胸腺小体明显减少；血浆ＡＣＴＨ、ＣＯＲＴ以及淋巴细胞增殖反应、白细胞介素２（ＩＬ－２）、γ－干扰素（γ－ＩＦＮ）诱生能力等降低。命门合剂灌胃能有效地改善ＣＯＲＴ对ＨＰＡＴ的抑制，Ｐ＜０．０１。提示命门合剂可能是调节ＨＰＡＴ轴的有效方剂。

The hypothalamus-pituitary-adrenal-thymus (HPAT) axis is an important form of the neuroendocrino-immunological network (NEIN).To study the essential link between Ming-Men,Kidney and the NEIN, the corticosterone (CORT) injected rats was used. The CORT caused HPAT axis inhibiting model was established and then the regulation of Ming-Men mixture (MMM) was observed. The results showed that the number of corticotropin-releasing factor (CRF) positive neurones in hypothalamic paraventricular nucleus, CRF positive neurofibril in median eminence,anterior pituitary ACTH positive secretory cells decreased markedly in the CORT inhibited rats and the immunohistochemical ABC staining became lighter than the control. We also observed that there was atrophy in adrenal,especially fasculate zone became thinner compared with the control.The thymus atrophied, the number of lymphocytes and thymic corpuscles decreased obviously. The content of ACTH and CORT in plasma decreased, the proliferative reaction of lymphocytes, inter leukin-2 and the inductive ability of γ-IFN reduced. After the MMM was instilled into stomach in experimental group,it was found that MMM could improve the inhibition on HPAT axis caused by CORT significantly, P<0. 01. It suggested that MMM possibly was an effective and ideal prescription which could regulate the HPAT axis.