比较黄芪和活血方对兔脑再灌注损伤血栓素A_2及前列环素和环核苷酸的影响

Comparison of Effect of Astragalus membranaceus and Huoxuefang on Thromboxane, Prostacyclin and Adenosine Cyclic Monophosphate in Cerebral Reperfusion Injury in Rabbits

兔脑缺血３０ｍｉｎ再灌注４５ｍｉｎ后，脑内血栓素Ｂ_２（ＴＸＢ_２）升高和６－酮－前列腺素Ｆ_（１α）（６－酮－ＰＧＦ_（１α））含量降低（Ｐ＜０．０５），血浆和脑环磷酸腺苷（ｃＡＭＰ）含量升高（Ｐ＜０．０１和Ｐ＜０．０５）。于缺血前分别静脉注射黄芪（３．３ｇ／ｋｇ）、活血方（２．５ｇ／ｋｇ）及益气活血方（４．４ｇ／ｋｇ），发现活血方和益气活血方均可抑制再灌注后脑ＴＸＢ_２的升高，提高脑６－酮－ＰＧＦ_（１ａ）含量（Ｐ＜０．０５）。活血方还可抑制脑ｃＡＭＰ的升高（Ｐ＜０．０５），黄芪则对此无明显影响。提示益气活血法通过改善脑局部ＴＸＡ２／ＰＧＩ_２平衡而起作用，主要为活血药物作用。

The cerebral ischemia rabbit model was made by using the occlusion of four vessels. The results showed that TXB_2 and cAMP contents in brain tissues and the latter in plasma markedly increased (P<0. 05, P<0 . 01), the 6-keto-PGF_la in brain tissues significantly lowered (P<0 . 05) in ischemia formed 30 minutes and 45 minutes after reperfusion. After intravenous injection of Astragalus membranaceus (AM) extracts (3.3g/kg), Huoxuefang (HXF) and Yiqi Huoxue Fang (YQHXF)consisted of AM and HXF before ischemia,the marked increase of TXB_2 contents after reperfusion was inhibited (P<0. 05) and the 6-keto-PGF_la in brain tissues after reperfusion were increased (P<0. 01) in HXF and YQHXF group, which change the AM extracts didn't have (P<0. 05). HXF could markedly inhibit the increase of cAMP in brain tissues after reperfusion (P<0.05),while the AM extracts and YQHXF couldn't (P>0 . 05).All above-mentioned suggested that the balancedisorder of TXA_2/PGI_2 in brain tissues might participate in the occurrence of cerebral reperfusion injury and YQHXF might act against this injury by means of improving the balance of TXA-2/PGI-2 in brain tissues, which was mainly released by HX drugs of it.