阿霉素诱导小鼠MCF-7乳腺癌细胞凋亡和抑制增殖的体内实验研究

In vivo study on apoptosis and cell proliferation induced by adriamycin in mice with MCF-7 breast cancer

目的:探讨阿霉素对荷瘤鼠MCF7乳腺癌细胞凋亡和增殖的影响。方法:建立小鼠MCF7乳腺癌细胞模型,用TUNEL法和免疫组化法检测阿霉素作用下MCF7乳腺癌细胞的凋亡和PCNA表达的变化。结果:1.25mg·kg-1阿霉素的抑瘤率为43%。MCF7乳腺癌细胞的凋亡指数为1.93%,较荷瘤对照组的1.11%明显升高(P<0.05)。增殖指数为35.75%,较荷瘤对照组的56.38%明显下降(P<0.01)。结论:阿霉素有诱导小鼠MCF7乳腺癌细胞凋亡和抑制其增殖的作用。

AIM: To observe the apoptosis and cell proliferation induced by adriamycin in mice with breast cancer. METHODS: Therapeutic effect of 1.25 mg·kg -1 adriamycin (ADR) on mice MCF-7 breast cancer was observed in vivo. Apoptosis and PCNA of tumor cells were also studied by TUNEL and immunohistochemical methods. RESULTS: In the control group, the apoptotic cell index (AI) in tumor cell was 1.11% and PCNA index (PI) was 56.38%. In the ADR group, the rate of tumor inhibition was 43% and the AI was 1.93% (P< 0.05). The 35.75% of PI in ADR group was lower than that in the control group (P< 0.01). CONCLUSION: Adriamycin may induce apoptosis and reduce cell proliferation.