摘要
AIM The aim of this study is to investigate the possible correlation between the expression of Nurrl gene and selective cell death of dopamine (DA) neurons. Nurrl ( nuclear receptor-related factor 1 ) is highly expressed in mesencephalic DA system and specifically required for development and survival of DArgic neurons, the cells primarily lost and aggressive degenerated in Parkinson’s disease (PD). METHODS Firstly, SK-N-SH cells, which do not express endogenous Nurrl, were transfected with the pBK-RSV-Nurrl plasmid by LipofectAMINE and selected by G418. The expression of Nurrl protein in SK-N-SH/Nurrl cells was determined by immunocytochemistry. The effect of Nurrl gene on cell proliferation was also investigated.
