肺炎衣原体感染对慢性阻塞性肺病患者T淋巴细胞亚群的影响

Effect of Chlamydia Pneumoniae Infection on T lymphocyte Subpopulations in Patients with Chronic Obstructive Pulmonary Disease

目的:探讨肺炎衣原体(CP)感染对慢性阻塞性肺病(COPD)患者T淋巴细胞亚群的影响。方法:选择COPD急性加重期患者176例,正常对照组46例。采用微量免疫荧光试验检测血清CP抗体IgG、IgA、IgM,采用流式细胞仪测定两组的外周血CD3+、CD4+、CD8+T淋巴细胞。结果:观察组急性CP感染率为27.3%,慢性CP感染率为19.3%,与对照组比较有显著差异(P<0.01)。CD3+、CD4+细胞在各组间无差异,COPD患者中慢性CP感染组的CD8+细胞升高、CD4+/CD8+比值降低,较对照组有明显差异(P<0.01),而急性CP感染组和阴性组的CD8+细胞及CD4+/CD8+比值与对照组差异不明显。结论:CP感染是COPD急性加重的重要诱因,CP感染特别是慢性感染是引起COPD患者细胞免疫功能紊乱的重要原因,而这种细胞免疫功能的紊乱可能参与了COPD的发病。

Objective:To explore the effect of chlamydia pneumoniae(CP) infection on T lymphocyte subsets in patients with chronic obstructive pulmonary disease(COPD).Methods:176 patients with acute exacerbations of COPD and 46 healthy subjects were selected.Chlamydia pneumoniae specific serum antibodies IgG,IgA and IgM were measured by microimmunofluorescence(MIF) test;flow cytometry was used to analyze the frequencies of CD3+,CD4+ and CD8+lymphocytes in patients with COPD and controls.Results:The rate of acute chlamydia pneumoniae infection in COPD patients with acute exacerbations was 27.3%;the rate of chronic CP infection was 19.3%.They were all significantly higher than in controls(P<0.01).The frequencies of the CD3+and CD4+ lymphocytes showed no difference between the two groups.The frequency of CD8+lymphocyte in COPD patients with chronic CP infection rose and the CD4+/CD8+ratio was decreased.These two parameters were significantly different from controls.There no significant difference in CD8+lymphocytes and the CD4+/CD8+ratio between COPD patients with chronic CP infection and without CP infection group,and controls.Conclusions:Chlamydia pneumoniae infection was regarded as an important precipitating cause of acute exacerbations of COPD.CP infection,especially chronic CP infection was major cause for cellular immunity disturbance in COPD patients.And this disturbance might be involved in pathogenesis of COPD.