胰腺炎相关腹水对大鼠胃肠动力影响

The Effect on Rat’s Gastrointestinal Motility of Pancreatitis-associated Ascite fluid

目的探讨急性胰腺炎相关腹水和L-NNA对离体胃肠平滑肌运动的影响。方法以5%牛磺胆酸钠溶液逆行注入胰胆管制作AP模型,24小时后收集腹腔液低温离心取上清液作为胰腺炎相关腹水。用Maclab数据分析系统,描记离体肠断的运动。观察20%和40%浓度的胰腺炎相关腹水单独和同时与L-NNA应用对离体空肠段运动的影响。结果20%和40%浓度的胰腺炎相关腹水可引起离体空肠运动峰值频率降低和平均振幅减弱,与自身基准组比较有显著性和非常显著性差异(P<0.05和P<0.01)。10-4M的L-NNA引起离体空肠平均振幅增加,差异有显著性意义(P<0.05),10-4M的L-NNA可逆转20%PAAF对离体肠管运动频率和振幅的影响,同时应用10-4M的LNNA和20%PAAF前后无显著性差异(P>0.05)。结论急性胰腺炎相关腹水抑制胃肠蠕动是通过降低胃肠平滑肌的运动频率和振幅,这种作用随腹水的浓度增高而增加。一氧化氮合酶抑制剂L-NNA主要增加大鼠肠动力,可逆转腹水的这种作用。腹水可能通过增加胃肠组织内NOS活性起作用。

Objective To investigate the effect on gastrointestinal motility of pancreatitis-associated ascite fluid and LNNA.Methods AP was produced with 5% sodium taurocholate by the method of retrograde pancreatic-injection.At 24hr after the induction of pancreatitis,the rats were sacrificed and the peritoneal exudates were aseptically collected followed by centrifugation at 40C.The supernatants from the rats were combined which were designated as pancreatitis-associated ascitic fluids (PAAF).We completed directly A/D change and achieved the computer real-time recording by combining the traditional recording device with Maclab/4e date recording and analysis systems.The effect of 20%and 40%PAAF and 10 -4 M L-NNA on the motility of the rat jejunum section in vitro was studied.Results the motility frequency and motility amplitude of the rat jejunum section were inhibited significantly by 20%and 40%PAAF(P<0.05 and P<0.01),10 -4 M L-NNA can inhibit the effect of 20%PAAF on rat jejunum.Conclusions The motility frequency and motility amplitude of gastrointestinal could be inhibited by PAAF in vitro.LNNA can inhibit the effect of PAAF on rat jejunum; our results suggest that PAAF play role in the gastrointestinal dysmotility during acute pancreatitis,at least in part through the activation of NOS.