Cyclin D1蛋白表达与卵巢癌转移相关性研究

Association between Cyclin D1 protein expression and metastasis of ovarian cancer

目的:探讨细胞周期蛋白(CyclinD1)表达与卵巢癌转移的相关性和CyclinD1与P21WAF1、P53蛋白的关系。方法:应用蛋白免疫印迹技术(Westernblot)检测61例人卵巢癌组织及7例卵巢非癌组织中CyclinD1、P53及P21WAF1蛋白的表达。结果:卵巢癌组织中CyclinD1和P53蛋白表达率均明显高于卵巢非癌组织(P<0.05);P21WAF1蛋白表达卵巢癌组织低于卵巢非癌组织(P>0.05)。CyclinD1与P53及P21WAF1蛋白表达与卵巢癌组织类型无关(P>0.05)。P21WAF1蛋白表达与卵巢癌的临床分期相关(P<0.05),CyclinD1、P53蛋白表达与卵巢癌的临床分期无关(P>0.05)。CyclinD1、P53及P21WAF1蛋白表达与卵巢癌转移相关(P<0.05)。结论:CyclinD1蛋白表达与卵巢癌肿瘤转移相关,CyclinD1与P21WAF1、P53蛋白在卵巢癌发生、发展中可能有一定作用,但不起协同作用。

Objective:To investigate the association between Cyclin D1 and metastasis and determine the relationship between Cyclin D1 proteins expression and P21 WAF1?P53 proteins in ovarian cancer.Methods:Cyclin D1,P21 WAF1 and P53 proteins were detected from 61 ovarian carcinoma and 7 ovarian tissues without cancer by using Western blot method.Results:The positive rate of Cyclin D1 and P53 proteins expression in cases with ovarian cancer were significantly higher than those in without cancer tissues(P<0.05),the expression of P21 WAF1 protein was not.No significant correlation were found between expression of Cyclin D1,P53 and P21 WAF1 proteins and histological grade(P>0.05).However, the expression of P21 WAF1 protein was correlated with clinical stages(P<0.05), but Cyclin D1 and P53 proteins were not(P>0.05).The expression of Cyclin D1,P53 and P21 WAF1 protein in ovarian carcinoma were related to metastasis(P<0.05).Conclusion:The expression of Cyclin D1 protein was correlated with tumor metastasis in ovarian cancer.Cyclin D1 and P21 WAF1,P53 proteins may play a role in the formation and development in ovarian cancer, but they may not act cooperatively in ovarian cancer.