牛肠毒素性产气荚膜梭菌的分离及鉴定

Isolation and Identification of Bovine Enterotoxigenic Clostridium Perfringens

对黑龙江省3个地区牛场疫牛进行临床诊断,剖检结果为小肠出血性肠炎和心内外膜严重出血等症状,进行了细菌的分离培养、涂片镜检、生化试验和动物感染试验。结果表明:分离菌株均为革兰氏阳性粗短杆菌,严格厌氧,在血平板上具有双溶血环,能发酵葡萄糖、麦芽糖、蔗糖和暴酵牛乳,肠毒素检测试验阳性,厌气肉肝汤培养物0.2mL小白鼠尾静脉注射,2～10h可使其致死。试验结果表明3个分离株均为产气荚膜梭菌。

A lot of dairy cattle and scalper suddenly died in some farms of Heilongjiang province,and their symptom in common was small intestinal bleeding of enteritis and bleeding of cardiac muscle after dissection. It was an acute epidemic disease. We made sure that the pathogenic bacteria was Cl.perfringens by collecting the pathogenic organs and tissues, isolating and culturing the bacteria, microscopy,biochemical test,animal infectious test and so on. We had filtered out more effective medicine which was effective for curing the pathogenic cattle and controlling the epidemic situation.