TNF-α和IFN-γ对OSF成纤维细胞胶原合成的影响

Effects of tumor necrosis factor-α and interferon-γ on collagen biosynthesis of OSF-derived fibroblasts

目的研究TNF-α、IFN-γ对正常人颊黏膜成纤维细胞(NM-FB)和口腔黏膜下纤维化(OSF)患者颊黏膜成纤维细胞(OSF-FB)胶原合成的影响.方法取6例OSF患者及5例正常人颊黏膜组织,采用组织块法进行成纤维细胞原代培养并传代,用羟脯氨酸试剂盒观察不同浓度TNF-α及IFN-γ对NM-FB和OSF-FB胶原合成的影响.结果浓度为100～10000 U/mL的TNF-α能明显促进NM-FB和OSF-FB的胶原合成(P＜0.05),浓度为40～4000U/mL r IFN-γ则明显抑制两者的胶原合成(P＜0.05),且400U/mL的IFN-γ其抑制率接近峰值(与4 000U/mL的IFN-γ组比,P＞0.05).结论TNF-α促进NM-FB和OSF-FB的胶原合成,而IFN-γ对两者的胶原合成具有抑制作用.

[Objection] To investigate the effects of tumor ne crosis factor-α (TNF-α) and interferon-γ (IFN-γ) on collagen biosynthesis of fibroblasts from normal persons(NM-FB) and patients with oral submucous fibrosis (OSF-FB). [Methods] Fibroblast cultures were established from the buccal mucosa of five normal person and six patients with OSF. The study focused on the effects of TNF-α and IFN-γ on the collagen biosynthesis of NM-FB and OSF-FB. [Results] TNF-α at 100~10 000 U/mL inhibit the collagen biosynthesis of NM-FB and OSF-FB, and they were reduced by IFN-γ at 40~4 000 U/mL. [Conclusions] The authors believe that TNF-α can upregulate and IFN-γ can deregulate the progression of OSF.